C-filter labels with DS-32 settings

Here you find screenshots from C-filter labels (FAM, HEX,TET) analyzed with DS-32 spectral, an unsupported combination.

Supported DS-32 run would be FAM, JOE and NED with ROX labeled size standard.
In this example run we analyzed FAM, HEX, and TET labeled fragments in a DS-32 run.

Dye	Looks like...	Comments
FAM analyzed with DS-32 filter Size standard: GS 500 LIZ	Click to zoom in	FAM also part of DS-32 set, no problems here. FAM = blue LIZ standard seen in red channel (ROX)
HEX analyzed with DS-32 filter Size standard: GS 500 LIZ	Click to zoom in	LIZ and HEX visible in FAM channel (blue) HEX visible in JOE channel (green) HEX slightly disturbs NED channel (black/yellow) LIZ standard seen in ROX channel (red)
TET analyzed with DS-32 filter Size standard: GS 500 LIZ	Click to zoom in	TET visible in FAM channel (blue) TET visible in JOE channel (green) TET interferes with NED (black/yellow) LIZ standard seen in ROX channel (red) Some LIZ signal also seen in FAM channel (blue)
Conclusions: FAM, HEX and TET are not compatible together in filter DS-32 run! HEX alone OK - use green channel in GeneMapper TET alone OK - use blue or green channel in GeneMapper

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Last modified: 09/10/08.