Quantitation of genomic DNA before PCR.
Before PCR amplification it is recommended to
check the concentration of your DNA samples. Uniform quality and concentration
of your DNA samples increases the success rate of PCR amplification and
subsequent sequencing reactions. SeqLab customers are welcome to
quantitate their DNA samples with
NanoDrop ND-1000 UV/Vis Spectrophotometer.
Nanodrop ND-1000 requires 1.0 - 1.5 µl of sample and measures concentration
within 10 seconds. Dynamic range of measurement is approximately
from 4 to 4000 ng/µl.
Remember to use proper blank sample to calibrate the
NanoDrop instrument.
Quantitation of plasmid DNA.
Plasmid purification kits and protocols may
yield very different concentrations of DNA. SeqLab welcomes you to measure your
plasmids with NanoDrop ND-1000 UV/Vis
Spectrophotometer.
Nanodrop ND-1000 requires only 1.5 µl of
sample and measures concentration within 10 seconds. Dynamic range of measurement is approximately
from 4 to 4000 ng/µl.
Remember to use proper blank sample to calibrate the
NanoDrop instrument. |