• Double click the PNA icon. Logon window opens.
• Fill in your username, password and domain "LTDK". Click "OK"
• Wait couple of seconds - Citrix PNA icon turns white indicating successful login
• Proceed to Start SequencingBrowser.
• Did you get errors?
  • Is certificate installed? If not go here.
  • Is domain correct (LTDK)
  • Is CapsLock on?

• Right click PNA icon - application menu opens.
• Select SequencingBrowser from folder "SeqLab"
• If SeqLab folder is not located in your menu, the "Med Genetics Seq Lab" option was not selected in your GIU application
  • Contact GIU administrator Juri Ahokas.

Sequencing Downloads

• Click "Sequencing Datas" tab.
• Copy '{Search Key}' from the automatic e-mail you received. '{Search Key}' is specific to a certain batch of samples you have submitted for us.
• Paste '{Search Key}' next to search button.
• Click "Search" and wait.
• Sequences corresponding to given '{Search Key}' are displayed.

NOTE: You may also use storage dates, sequencing names etc to restrict your search.

• Click "Extract all sequence files"
• Sequence format - window opens
  • Select "Extract original trace files to a folder" and click OK.
• "Save as" window opens
  • Select my Computer from "Save in" drop-down menu
  • Your local C:-drive is shown as "C$ on 'Client' (V:) - see picture
  • Browse for your destination folder and click "Save"
• ABI chromatogram files will be written to the selected folder.

• Click "Fragment analysis runs" tab.
• Select search criteria (date, run name etc)
• Click "Search"
• Click "Extract all fragment files" or
• "Extract selected fragment file" (only one run)
• Select destination folder and click "OK"
• Fragment analysis run will be saved as a ZIP-file in selected folder.
• UnZip run using WinZip program.
• Analyze using GeneMapper or GeneMarker.