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Evaluate sequencing results
It is recommended to review the quality of your sequencing
chromatograms before you use the sequences.
Some sequence chromatogram viewers and analysis software is listed in
"Software Links" section. |
SequenceScanner v1.0 (Applied Biosystems) enables you to
quickly view, edit, print and export sequence data generated using the Applied
Biosystems Genetic Analyzers. The software helps you to:
- Review all traces in thumbnail format and sort them by
trace quality.
- View simultaneously the analyzed and raw trace data for a
single trace.
- Spot sample failures readily with reports such as the Plate
Report.
- Export traces into commonly used presentation formats
(.jpeg, pdf).
SequenceScanner quick help:
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Download and install SequenceScanner
- Start SequenceScanner
- Select "File - Import traces"
- Select the folder containing chromatograms - click "OK"
- All chromatograms within the selected folder are opened
- For example select "View - Thumbnails" to see miniature raw data
profiles
- Double-click on any sequence - a chromatogram window opens
- Select "View - Reports" from the menus - Reports section opens.
- Browse through reports - print or export to Excel if needed.
- For example "Plate report" gives you quick overview of the
location of your samples on the plate and how well reactions succeeded.
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Some screenshots from SequenceScanner:
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View sequencing chromatograms |
View sequence raw data profiles |
View plate reports |
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View read length report |
View & export QC report |
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