Fragment Analysis Run |
|
|
Fragment analysis run is a
ABI3730xl electrophoresis run of 96 samples - a full plate is
analyzed in a single run.
Standard fragment analysis run uses G5 dye set (6-FAM, VIC, NED,
PET with LIZ reserved for internal size standard). With a standard setting for a Fragment Analysis
Run a resolution of 1 bp is
achieved up to 500 bp with GeneScan-500
LIZ size standard. Please contact
SeqLab staff if you wish to use other dyes or other size standards.
NOTE: A novel 1200 bp protocol is available with GeneScan LIZ-1200 size standard. NOTE: If you are setting up a new fragment analysis assay please read first Assay design / testing. About fluorescent dyes
|
 |
|
|
|
Fragment Analysis Run includes: |
|
Customer provides: |
|
Preparation of a Fragment Analysis Run plate |
||
| Prepare a master mix of Hi-Di Formamide and GeneScan LIZ-500 size standard (or other): | ||
|
Component | per well |
| Hi-Di | 10 µl | |
| LIZ-500 | 0.025 µl | |
| PCR (diluted) | 2.0 µl | |
| NOTE: Keep the amount of size standard constant from assay to assay - it is enough for successful Fragment Analysis Run. Adjust the dilution of your PCR products in the sample if necessary to gain balanced PCR:size standard ratio. | ||
| NOTE: Fill each well at
least with Hi-Di Formamide. Empty well may lead to capillary damage when
injecting with high voltage. If you have empty wells in the run,
fill them with duplicates or other dilutions of your PCR fragments. |
||
Submit order & Deliver samples
|
||