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Service Types

Service types

Sequencing laboratory provides three categories of services:
Full Service sequencing Ready-to-Run sequencing Fragment Analysis run

Full Service sequencing
Sample batches of 96 samples require processing time of about 2 working days (If samples and Sample submission form delivered before 10:00 in the morning). Individual samples (i.e. any other than full series or 96) may take longer. Individual samples are also charged with higher price. Expected read length (QV>20) for high quality DNA template/primer is approximately 550-600 bp.
Customer provides:

 

Full Service sequencing includes:
  • BigDye v.3.1 sequencing reaction for customer's template-primer cocktails.
  • Sequencing reaction purification: dye terminator removal with Performa DTR v3 filter plates.
  • Electrophoresis with ABI3730xl DNA Analyzer.
  • Basecalling with Sequencing Analysis 5.2
  • Storage of chromatograms into SeqLab database.
  • Automatic e-mail notification when results are saved to database
    • including batch specific Search Key for easy access to results.
See protocol for Full Service sequencing.
Please fill plates by columns, click picture to enlarge.

Ready-to-Run sequencing
Ready-to-Run sample batches of  96 require processing time of about 1-2  working days if samples and Sample submission form delivered before 10:00 in the morning. Individual samples (i.e. any other than full series of 96) may take longer. Expected read length (QV>20) for high quality DNA template/primer is approximately 550-600 bp.

Customer provides:

  • Ready made sequencing reactions (10µl) preferably in a 96-well plate format.
  • Completely filled Sample submission form as e-mail attachment sent to sequencing(at)ktl.fi.
Ready-to-Run sequencing includes:
  • Sequencing reaction purification: dye terminator removal with Performa DTR v3 filter plates.
  • Electrophoresis with ABI3730xl DNA Analyzer.
  • Basecalling with Sequencing Analysis 5.2
  • Storage of chromatograms into SeqLab database.
  • Automatic e-mail notification when results are saved to database
    • including batch specific Search Key for easy access to results.

See protocol for Ready-to-Run sequencing.
Please fill plates by columns, click picture to enlarge.

Fragment Analysis run
Fragment analysis run is a ABI3730xl electrophoresis run of 96 samples - the whole plate is analyzed with a single run. Standard fragment analysis run uses G5 dye set (6-FAM, VIC, NED PET with LIZ size standard). With a standard setting for a Fragment Analysis Run a resolution of 1 bp is achieved up to 500 bp with GeneScan-500 LIZ size standard. Please contact SeqLab staff if you wish to use other dyes, other size standards or fragments longer than 500 bp.

Customer provides:

  •  Ready-to-run samples in Hi-Di formamide including size standard on ABI 96-well Optical reaction plate with barcode
Fragment Analysis run includes:
  • Electrophoresis with ABI3730xl DNA Analyzer.
  • Storage of electrophoresis run as a ZIPped file into a database.
  • Automatic e-mail notification when results are saved to database.
See protocol for Fragment Analysis run.
Please fill always full plate, click picture to enlarge.

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Questions or problems regarding this web site should be directed to SeqLab.
Last modified: 09/10/08.