Sometimes it is practical to dry sequencing
templates and primers directly to the PCR plate. Dried samples are easy to
send by mail or courier without the fear of leakage or sample mix-up. Dried
samples can be stored in room temperature for days. When sending dried
samples please remember following:
- Use only V-bottom 96 well PCR
plate (e.g. ABGene AB-0800 or equivalent).
- We will add sequencing reagents
directly to the dried wells and perform sequencing reactions.
- U or flat bottom plates are not
accepted - they don't fit to our PCR thermocyclers.
For Full Service Samples:
- Pipette template and primer enough
for a SINGLE reaction per well (total 2,65 µl)
- Pipette 2 µl of DNA template
- Add 0,65 µl of 5 µM sequencing
primer
- Spin plate and dry as follows:
- Remove seal/caps from the plate
- Cover the plate loosely with
foil to protect samples
- Dry in an oven (e.g. 50ºC)
- or dry overnight in room
temperature.
- Seal the plate properly
- Mark the plate CLEARLY with text
"DRY FULL SERVICE".
- In sample submission form's
"Service type" field write DRY FULL SERVICE (or DRY FS)
- Proceed to
"Submit Order"
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