SeqLab's technical resources are listed here. Some instruments are available for our customers free-of-charge after proper training from SeqLab staff.

NOTE: Equipment room is post-PCR region (amplified DNA is handled in the room).
 

• High-throughput genetic analyzer
• 96-capillary electrophoresis instrument
• 16 plate hotel for walkaway automation
• BigDye 3.1 (and 1.1) sequencing chemistries
• G5 (DS-33) fragment analysis chemistry
• Software: DataCollection 3.0, Sequencing Analysis 5.2, GeneMapper 4.0
• More info www.appliedbiosystems.com
• Operated by SeqLab staff only.

• Full-spectrum UV-Vis absorbance analyses (220-750nm)for measuring absorbance of DNA, RNA etc.
• 1.5 μl of sample, 10 second measurement
• Large dynamic range: 2-3700 ng/ul of dsDNA
• No dilutions for most samples, no cuvettes.
• ND-1000 v3.3 Software with Data Viewer - User manual.
• More info http://www.nanodrop.com/
• Freely available after training - contact SeqLab staff.

• Public sequencing and fragment analysis PC
• 1 PC in HY network - 1 PC in KTL network.
• Analysis software freely available:
  • SequenceScanner v1.0 (ABI, freeware)
  • SequencingAnalysis 5.2 (ABI, license)
  • Sequencher 4.7 (GeneCodes, license)
  • Mutation Surveyor 3.0 (SoftGenetics, license)
  • SeqScape v2.5  (ABI, license)
  • FinchTV 1.4 (Geospiza, freeware)
  • Staden Package (freeware)
  • GeneMapper 4.0 (ABI, license)
  • GeneMarker1.51 (SoftGenetics, licence)
  • PeakScanner v1.0 (ABI, freeware)
• Software manuals are located next to the computers.

• For robust and quick plate sealing.
• Plate Compatibility:
  • The ALPS 300 seals skirted plates of differing heights without the need for plate adapters.
  • Adapter is only necessary to for non-skirted PCR plates.
• ABGene Clear Seal 3730 tape used for heat sealing.
• More info www.abgene.com
• Reserved for SeqLab use - contact staff for more information.

• Primarly for SeqLab use (plate purifications)
• Swinging bucket  rotor (7500 6445)
• Carrier for microplates, max 4 x 4 plates (7500 6449)
• Max 4600 rpm / 3760g with this configuration
• Imbalance detection system and automatic rotor recognition
• Programmable memory (4 + 1 pretemp program)
• Nine braking and acceleration profiles
• Refridgeration unit for cooling.
• Available for you if not used by SeqLab staff.

• 8 channel individual pitetting tool with fixed tips
• Te-Mo 96-channel replicator, disposable or fixed tips
• Tubes, 96well plates, 384 well plates, glass slides
• Used for post-PCR applications:
  • Sequencing reaction setup
  • Sequencing purification
  • DNA microarray pipetting
  • WGA reaction setup
   
• More info www.tecan.com
• If you need automated post-PCR liquid handling contact SeqLab staff.

• Check your PCR templates in 5 min

• Freely available for SeqLab customers

• Bring your own gel cassettes

• Contact SeqLab staff to access FlashGel instrument

• Features:

  • Get results in 5 minutes (up to 4 kb)

  • See DNA migrate in real time without UV light

  • The FlashGel System consists of precast agarose gel cassettes and a combination electrophoresis and transilluminator unit.

  • The FlashGel™ Dock is an electrophoresis apparatus with a built-in transilluminator that provides both separation and detection.

  • FlashGel™ Cassettes contain precast, prestained agarose gels and buffer.

• More info www.cambrex.com