• The genotyping is performed for batches of 16 samples.
• 250 ng of high quality DNA is needed for each sample (see DNA preparation help).
• Processing of samples takes 3 consecutive working days. Samples are required on Tuesdays.
• SeqLab provides quality controlled genotypes in Excel tables.

If you are interested please contact SeqLab staff.
 

Application

Mouse genome-wide genotyping using single nucleotide polymorphisms (SNPs).

Optimal for various mapping applications that include characterization of transgenic, congenic and knockout mice, for example when a homogeneous genetic background is needed.

 Advantage of using speed congenics strategy Traditionally, animals for congenic breeding have been selected arbitrarily without the knowledge of animals' genotype outside the transgene region. In speed congenic strategy, animals are genotyped with a genome-wide panel of genetic markers and animals with optimal genotype are selected for the next breeding step. This will speed backcrossing about two-fold.
 

Platform

The platform used for genotyping is Illumina's Mouse MD Linkage panel which consists of 1449 SNP markers. These SNPs have been chosen to maximize genetic information across ten inbred strains (129S1/SvImJ, AKR/J, BALB/cJ, C3H/HeJ, C57BL/6J, CBA/J, DBA/2J, FVB/NJ, NOD/LtJ, SJL/J). SNPs in this panel were chosen to first provide uniform genome distribution at a density of approximately 3 SNPs per 5 Mb intervals across the genome. On average, at least one SNP in each interval is informative for 75% of pairwise strain combinations. Detailed information about genotyping assay at Illumina's website.