
Filter plate PCR purification (EDGE Biosystems)
After PCR amplification your samples contain excess
deoxynucleotides and primers which were not consumed during amplification. Extra
nucleotides and primers has to be removed before sequencing reaction or they
will severely affect the quality of the results. (see picture).
We have evaluated and optimized a rapid PCR purification
protocol using resin and filter plates. Protocol is modified from EDGE
Biosystems Quickstep kit.
Coming soon...meanwhile ask SeqLab Staff
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